UEFA revealed on Friday (AEST) that the opening leg of the last-four encounter will be played on April 30 (May 1 AEST), with Barcelona taking on Liverpool at Camp Nou the following day.
Seven days later, last season's beaten finalists Liverpool host Barcelona in the first of the second legs before Ajax welcome Spurs to Amsterdam.
Atletico Madrid's Wanda Metropolitano home will then be the venue for the final of this season's Champions League on June 1 (June 2 AEST).
May 1: Tottenham v Ajax
May 2: Barcelona v Liverpool
May 8: Liverpool v Barcelona
May 9: Ajax v Tottenham
*All dates in AEST